{"id":1114,"date":"2018-09-24T19:19:03","date_gmt":"2018-09-24T19:19:03","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/csb\/?page_id=1114"},"modified":"2025-07-18T11:47:40","modified_gmt":"2025-07-18T16:47:40","slug":"cryo-em-equipment","status":"publish","type":"page","link":"https:\/\/www.vanderbilt.edu\/csb\/facilities\/cryo-electron-microscopy\/cryo-em-equipment\/","title":{"rendered":"Cryo-EM Equipment"},"content":{"rendered":"<h2>Equipment and Resources<\/h2>\n<p>Visit the facility <a href=\"https:\/\/vanderbilt.corefacilities.org\/service_center\/5748\/\">iLab site<\/a> for equipment reservations, training, and consultation requests.<\/p>\n<p>Information to set up an iLab Account: <a title=\"Original URL: https:\/\/www.vanderbilt.edu\/cores\/Setting_Up_an_iLab_User_Account_Guide.pdf Click to follow link.\" href=\"https:\/\/nam04.safelinks.protection.outlook.com\/?url=https%3A%2F%2Fwww.vanderbilt.edu%2Fcores%2FSetting_Up_an_iLab_User_Account_Guide.pdf&amp;data=04%7C01%7Cmelissa.g.chambers%40¹ú²ú´«Ã½.Edu%7C9435a8971b484dde261e08d91fbfa36b%7Cba5a7f39e3be4ab3b45067fa80faecad%7C0%7C0%7C637575730198863609%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&amp;sdata=AeU5SxZ3ra6og4o%2FDd7uu4SedoSYYoNdXDRIcNZRQVQ%3D&amp;reserved=0\" target=\"_blank\" rel=\"noopener\">iLab Account Basics and\u00a0Setting Up a New User Account Guide<\/a>.<\/p>\n<h4>Microscopes<\/h4>\n<ul>\n<li><span style=\"color: #37638c\"><strong>Titan Krios G4<\/strong><\/span>, 300kV TEM with Gatan K3 BioQuantum and ThermoScientific Falcon3 direct electron detectors: for collecting large dataset of high-resolution cryo single particle and tomography data<\/li>\n<li><strong><span style=\"color: #37638c\">Glacios<\/span><\/strong>, 200kV TEM with Falcon4i direct electron detector: for screening vitrified grids and collecting small data sets of high-resolution cryo single particle, tomography, and MicroED data<\/li>\n<li><span style=\"color: #37638c\"><strong>L120C<\/strong><\/span>, 100kV TEM with Ceta-S 4k x 4k CMOS detector: for screening and collecting negative stain data<\/li>\n<\/ul>\n<h4>Auxiliary<\/h4>\n<ul>\n<li>2 ThermoScientific Vitrobots, Mark II and Mark IV<\/li>\n<li>1 Leica EM GP2 plunge freezer<\/li>\n<li>2 Gatan 626 cryo-transfer holders<\/li>\n<li>2 Carbon evaporators<\/li>\n<li>2 Glow dischargers<\/li>\n<li>GPU workstations<\/li>\n<\/ul>\n<h4>Other on-campus equipment<\/h4>\n<ul>\n<li><span style=\"color: #333399\"><strong><span style=\"color: #37638c\">Helios NanoLab G3 CX Dual Beam FIB-SEM with Quorum PP3010T Cryo System: <\/span><\/strong><\/span>for preparing lamella for TEM imaging and other capabilities.\u00a0 See <a href=\"https:\/\/www.vanderbilt.edu\/vinse\/instrumentation\/focused-ion-beam\/\" target=\"_blank\" rel=\"noopener\">VINSE<\/a> website for more info.<\/li>\n<li><span style=\"color: #333399\"><strong><span style=\"color: #37638c\">Tecnai G2 Osiris S\/TEM: <\/span><\/strong><\/span>for imaging of purified and thin sectioned samples and EDS element detection.\u00a0 See <a href=\"https:\/\/www.vanderbilt.edu\/vinse\/instrumentation\/transmission-electron-microscope\/\" target=\"_blank\" rel=\"noopener\">VINSE<\/a> website for more info.<\/li>\n<li><span style=\"color: #666699\"><strong><span style=\"color: #333399\"><span style=\"color: #37638c\">JEOL TEM 2100 Plus TEM:<\/span><\/span><\/strong><\/span> for imaging of purified and thin sectioned samples.\u00a0 See <a href=\"https:\/\/my.vanderbilt.edu\/cisr\/services-and-equipment\/electron-microscopy\/\">CISR website<\/a> for more info.<\/li>\n<li><span style=\"color: #666699\"><strong><span style=\"color: #333399\"><span style=\"color: #37638c\">Zeiss Crossbeam 550 FIB-SEM:<\/span><\/span><\/strong><\/span>\u00a0for preparing lamella for TEM imaging and other capabilities.\u00a0 See <a href=\"https:\/\/my.vanderbilt.edu\/cisr\/services-and-equipment\/electron-microscopy\/\">CISR website<\/a> for more info.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h4><\/h4>\n","protected":false},"excerpt":{"rendered":"<p>Equipment and Resources Visit the facility iLab site for equipment reservations, training, and consultation requests. Information to set up an iLab Account: iLab Account Basics and\u00a0Setting Up a New User Account Guide. Microscopes Titan Krios G4, 300kV TEM with Gatan K3 BioQuantum and ThermoScientific Falcon3 direct electron detectors: for collecting large dataset of high-resolution cryo&#8230;<\/p>\n","protected":false},"author":1185,"featured_media":0,"parent":128,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"spay_email":""},"tags":[],"acf":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages\/1114"}],"collection":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/users\/1185"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/comments?post=1114"}],"version-history":[{"count":21,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages\/1114\/revisions"}],"predecessor-version":[{"id":6148,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages\/1114\/revisions\/6148"}],"up":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/pages\/128"}],"wp:attachment":[{"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/media?parent=1114"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/csb\/wp-json\/wp\/v2\/tags?post=1114"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}