{"id":626,"date":"2018-07-09T13:10:36","date_gmt":"2018-07-09T19:10:36","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/cit\/?page_id=626"},"modified":"2025-04-09T12:00:14","modified_gmt":"2025-04-09T18:00:14","slug":"available-omics-instrumentation","status":"publish","type":"page","link":"https:\/\/www.vanderbilt.edu\/cit\/available-omics-instrumentation\/","title":{"rendered":"Available Omics Instrumentation"},"content":{"rendered":"<div class=\"flex_column av_three_fourth  flex_column_div av-zero-column-padding first  avia-builder-el-0  el_before_av_one_fourth  avia-builder-el-first  \" style='border-radius:0px; '><p><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p>The CIT\u00a0houses state-of-the-art omics instrumentation and technology and our multi-vendor approach allows for <strong>highly customizable, flexible applications<\/strong> that can be tailored to our client&#8217;s specific needs.<\/p>\n<p>Instrumentation includes:<\/p>\n<ul>\n<li>four LC-ion mobility mass spectrometers<\/li>\n<li>one high resolution LC-Orbitrap mass spectrometer<\/li>\n<li>one LC-QqQ (triple quadrupole) mass spectrometer<\/li>\n<\/ul>\n<p>This omics instrumentation is supported by a variety of sample handling accessories including autosamplers, ultra high performance liquid chromatography (UHPLC), supercritical fluid chromatography (SFC), and a nanoflow ChipCube platform.<\/p>\n<p>Importantly, the CIT (in conjunction with the <a href=\"http:\/\/www.vanderbilt.edu\/AnS\/Chemistry\/groups\/mcleanlab\/\" target=\"_blank\" rel=\"noopener\">McLean laboratory)<\/a>\u00a0is\u00a0designated as a\u00a0<strong>Waters Center of Innovation<\/strong> (Waters Corporation) and recognized as an <strong>Agilent Thought Leader<\/strong> laboratory (Agilent Technologies).<\/p>\n<\/div><\/section><br \/>\n<div  style='height:30px' class='hr hr-invisible   avia-builder-el-2  el_after_av_textblock  avia-builder-el-last '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div><\/p><\/div>\n<div class=\"flex_column av_one_fourth  flex_column_div av-zero-column-padding   avia-builder-el-3  el_after_av_three_fourth  el_before_av_textblock  \" style='border-radius:0px; '><div  class='avia-image-container  av-styling-    avia-builder-el-4  avia-builder-el-no-sibling  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='wp-image-116 avia-img-lazy-loading-not-116 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223105\/vials-158x300.jpg\" alt='Mass Spectrometry Sample Vials' title='Mass Spectrometry Sample Vials' height=\"300\" width=\"158\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223105\/vials-158x300.jpg 158w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223105\/vials.jpg 300w\" sizes=\"(max-width: 158px) 100vw, 158px\" \/><\/div><\/div><\/div><\/div>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p><strong>Liquid chromatography-mass spectrometry (LC-MS) tandem instruments<\/strong> offer the ability to separate compounds by both polarity (LC) and mass-to-charge (MS) ratio dimensions. A range of chromatography methods including reverse phase liquid chromatography (RPLC) and hydrophillic interaction liquid chromatography (HILIC) can be used to optimize stationary phase retention.<\/p>\n<\/div><\/section>\n<div class=\"flex_column av_one_full  flex_column_div av-zero-column-padding first  avia-builder-el-6  el_after_av_textblock  el_before_av_hr  column-top-margin\" style='border-radius:0px; '><div  class='avia-image-container  av-styling-    avia-builder-el-7  avia-builder-el-no-sibling  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='wp-image-844 avia-img-lazy-loading-not-844 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2018\/07\/09130307\/Omics-Instrumentation_1000.jpg\" alt='' title='Omics-Instrumentation_1000' height=\"512\" width=\"1000\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2018\/07\/09130307\/Omics-Instrumentation_1000.jpg 1000w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2018\/07\/09130307\/Omics-Instrumentation_1000-300x154.jpg 300w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2018\/07\/09130307\/Omics-Instrumentation_1000-768x393.jpg 768w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2018\/07\/09130307\/Omics-Instrumentation_1000-705x361.jpg 705w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2018\/07\/09130307\/Omics-Instrumentation_1000-450x230.jpg 450w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/div><\/div><\/div><\/div>\n<div  style='height:50px' class='hr hr-invisible   avia-builder-el-8  el_after_av_one_full  el_before_av_tab_container '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div>\n<div  class=\"tabcontainer   top_tab   avia-builder-el-9  el_after_av_hr  el_before_av_hr \" role=\"tablist\">\n\n<section class=\"av_tab_section\"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div aria-controls=\"tab-id-1\" role=\"tab\" tabindex=\"0\" data-fake-id=\"#tab-id-1\" class=\"tab active_tab\"  itemprop=\"headline\" >LC-IM-MS<\/div>\n<div id=\"tab-id-1\" class=\"tab_content active_tab_content\" aria-hidden=\"false\">\n<div class=\"tab_inner_content invers-color\"  itemprop=\"text\" >\n<p>The CIT houses\u00a0several commercially available <strong>liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS)<\/strong> instruments. These instruments offer the ability to separate ions or metabolites in three dimensions: polarity (LC), molecular size\/shape\/charge (IM), and mass-to-charge (MS). All instruments are optimized for sensitivity to low molecular weight analytes (&lt;2000 Da).<\/p>\n<p>Both\u00a0<strong>traveling wave<\/strong> ion mobility-mass spectrometry (IM-MS) instruments (Synapt G2 HDMS and Synapt G2-S HDMS models, Waters Corporation) are coupled to Acquity UPLC or nanoAcquity UPLC systems. The G2-S HDMS model incorporates StepWave ion transfer optics and TriWave IM technologies for increased sensitivity. Both, Synapt nano- and conventional electrospray ionization (ESI) utilize a lockspray, which allows for mass calibration during data acquisition. In addition, a quadrupole using a collision-induced dissociation (CID) cell both directly before and after the traveling wave mobility cell can perform mass selection and fragmentation.<\/p>\n<p>The CIT also houses two state-of-the-art Agilent ion mobility instruments (Model 6560). These instruments were built with <strong>enhanced drift tube<\/strong> ion mobility resolution and precision. The 6560s are coupled to either a binary LC system\u00a0(1260 Infinity, Agilent) or a nano flow LC (Chip Cube capabilities) that utilizes columns or prepackaged chips (Chip Cube) for enhanced reproducibility. The dual jet stream source is an electrospray ionization (ESI) source for increased sensitivity capabilities. For more specialized analyses, the static field ion mobility cell is capable of running in numerous gas conditions (i.e., nitrogen, helium and carbon dioxide) depending on the user\u2019s needs.<\/p>\n<p><em>Example Applications: lipidomics, chiral separations, structural characterization, biological and environmental analyses, xenobiotics, exposomics, environmental health (water testing)<\/em><\/p>\n\n<\/div>\n<\/div>\n<\/section>\n\n<section class=\"av_tab_section\"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div aria-controls=\"tab-id-2\" role=\"tab\" tabindex=\"0\" data-fake-id=\"#tab-id-2\" class=\"tab \"  itemprop=\"headline\" >Orbitrap LC-MS<\/div>\n<div id=\"tab-id-2\" class=\"tab_content \" aria-hidden=\"true\">\n<div class=\"tab_inner_content invers-color\"  itemprop=\"text\" >\n<p>The CIT houses one state-of-the-art LC-HRMS omics instrument: the Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific). This instrument combines a segmented quadrupole for high-performance precursor ion selection with a <strong>high-resolution and accurate-mass (HR\/AM), ultra-high-field Orbitrap<\/strong> <strong>mass analyzer<\/strong> offering a combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity.<\/p>\n<p>Our\u00a0QE-HF is equipped with an <strong>ultra-high performance liquid chromatography system<\/strong> (Vanquish, ThermoFisher Scientific), <strong>nano- and conventional electrospray ionization (ESI)<\/strong> sources.<\/p>\n<p><em>Example Applications: drug metabolites, biotechnological samples, and environmental toxins\u00a0\u2013 even in complex matrices<\/em><\/p>\n\n<\/div>\n<\/div>\n<\/section>\n\n<section class=\"av_tab_section\"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div aria-controls=\"tab-id-3\" role=\"tab\" tabindex=\"0\" data-fake-id=\"#tab-id-3\" class=\"tab \"  itemprop=\"headline\" >Triple Quadrupole LC-MS<\/div>\n<div id=\"tab-id-3\" class=\"tab_content \" aria-hidden=\"true\">\n<div class=\"tab_inner_content invers-color\"  itemprop=\"text\" >\n<p>For <strong>targeted analysis<\/strong> experiments (mainly multiple reaction monitoring (MRM), dynamic MRM (dMRM), and triggered MRM (tMRM)), the CIT is utilizing an Agilent 6470 Triple Quadrupole (QqQ) LC\/MS system. The components of this tandem system include <strong>two mass filters and a collision cell,\u00a0enhanced ion optics and detector design<\/strong> over other systems allowing for <strong>attogram level sensitivity <\/strong>for specified analytes.<\/p>\n<p>Through its increased sensitivity, wider linear range, and better signal-to-noise ratios it allows for <strong>lower detection limits<\/strong>. This instrument can perform\u00a0<strong>either relative or absolute quantification<\/strong> of small molecules of interest.<\/p>\n<p>Generally, global untargeted analyses precedes targeted analysis experiments,<strong>\u00a0<\/strong>unless specific metabolic pathways or metabolites (i.e., TCA cycle, carnitine biosynthesis, amino acids, redox\/energy molecules) are of interest to the user.<\/p>\n<p><em>Example Applications:\u00a0metabolites, drugs, lipids, peptides, amino acids, and canonical metabolic pathways of interest for\u00a0biomarker validation, pharmaceuticals, steroids, toxicology\/environmental analyses, and forensics<\/em><\/p>\n\n<\/div>\n<\/div>\n<\/section>\n\n<\/div>\n\n<div  style='height:50px' class='hr hr-invisible   avia-builder-el-10  el_after_av_tab_container  el_before_av_one_fifth '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div>\n<div class=\"flex_column av_one_fifth  flex_column_div av-zero-column-padding first  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'><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":959,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/pages\/626"}],"collection":[{"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/users\/959"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/comments?post=626"}],"version-history":[{"count":36,"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/pages\/626\/revisions"}],"predecessor-version":[{"id":1632,"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/pages\/626\/revisions\/1632"}],"wp:attachment":[{"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/media?parent=626"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}