{"id":22,"date":"2015-12-02T10:02:04","date_gmt":"2015-12-02T16:02:04","guid":{"rendered":"http:\/\/www.vanderbilt.edu\/cit\/?page_id=22"},"modified":"2025-04-09T11:53:47","modified_gmt":"2025-04-09T17:53:47","slug":"mass-spectrometry-technology-services","status":"publish","type":"page","link":"https:\/\/www.vanderbilt.edu\/cit\/mass-spectrometry-technology-services\/","title":{"rendered":"Services"},"content":{"rendered":"<div class=\"flex_column av_one_third  flex_column_div av-zero-column-padding first  avia-builder-el-0  el_before_av_one_third  avia-builder-el-first  \" style='border-radius:0px; '><p><div  class='avia-image-container  av-styling- av-hover-grow   avia-builder-el-1  el_before_av_textblock  avia-builder-el-first  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='wp-image-110 avia-img-lazy-loading-not-110 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223108\/MassSpec2-495x335.jpg\" alt='Agilent Technologies at 国产传媒' title='Agilent Technologies at 国产传媒' height=\"335\" width=\"495\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center\">Agilent 6560 LC-IM-MS<\/p>\n<\/div><\/section><\/p><\/div>\n<div class=\"flex_column av_one_third  flex_column_div av-zero-column-padding   avia-builder-el-3  el_after_av_one_third  el_before_av_one_third  \" style='border-radius:0px; '><p><div  class='avia-image-container  av-styling- av-hover-grow   avia-builder-el-4  el_before_av_textblock  avia-builder-el-first  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='wp-image-111 avia-img-lazy-loading-not-111 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223107\/MassSpec3-495x335.jpg\" alt='Mass Spectrometry Shared Resource Collaboration' title='Mass Spectrometry Shared Resource Collaboration at 国产传媒' height=\"335\" width=\"495\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center\">Waters Synapt G2 LC-IM-MS and<br \/>\nWaters Synapt G2-S LC-IM-MS (not shown)<\/p>\n<\/div><\/section><\/p><\/div>\n<div class=\"flex_column av_one_third  flex_column_div av-zero-column-padding   avia-builder-el-6  el_after_av_one_third  el_before_av_hr  \" style='border-radius:0px; '><p><div  class='avia-image-container  av-styling- av-hover-grow   avia-builder-el-7  el_before_av_textblock  avia-builder-el-first  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='wp-image-109 avia-img-lazy-loading-not-109 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223109\/MassSpec1-495x335.jpg\" alt='Thermo Extractive HF' title='Thermo Extractive HF' height=\"335\" width=\"495\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p style=\"text-align: center\">Thermo Q-Exactive HF LC-MS<\/p>\n<\/div><\/section><\/p><\/div>\n<div   class='hr hr-default   avia-builder-el-9  el_after_av_one_third  el_before_av_three_fourth '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div>\n<div class=\"flex_column av_three_fourth  flex_column_div av-zero-column-padding first  avia-builder-el-10  el_after_av_hr  el_before_av_one_fourth  \" style='border-radius:0px; '><p><div id='avia-messagebox-' class='avia_message_box  avia-color-blue avia-size-large avia-icon_select-no avia-border-  avia-builder-el-11  el_before_av_iconlist  avia-builder-el-first '><div class='avia_message_box_content' ><p>The Center for Innovative Technology is a full-service molecular omics shared resource facility offering streamlined workflows, cutting-edge resources, and a collaborative approach that allows you to focus on what matters most.<\/p>\n<\/div><\/div><br \/>\n<div  class='avia-icon-list-container   avia-builder-el-12  el_after_av_notification  avia-builder-el-last '><ul class='avia-icon-list avia-icon-list-left av-iconlist-big avia_animate_when_almost_visible avia-iconlist-animate'>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue80b' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Consultation Services<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p>We are your local specialist in untargeted molecular omics and with over 50 years of combined experience in mass spectrometry, data analysis, and informatics pipelines, the CIT is happy to provide assistance in experimental design and fit-for-purpose analyses for your molecular omics applications.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue8c5' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Biostatistical Services<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p>The CIT works in collaboration with the <a href=\"https:\/\/www.vumc.org\/cqs\/\" target=\"_blank\" rel=\"noopener\">Center for Quantitative Sciences<\/a> at 国产传媒 to provide biostatistics, bioinformatics, systems biology, and outcome research support, incl. (advanced) sample size determination, power analyses, and multivariate modeling for high-dimensional data.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue8ba' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Sample Preparation<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p>Choosing suitable sample preparation, metabolic quenching, and extraction methods requires an understanding of the capabilities and limitations of each method for various sample types and applications. As a full-service facility, our experienced staff is there to take the guesswork out of it.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue804' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Untargeted Metabolomics<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p><strong>Global, untargeted metabolomics<\/strong>\u00a0is hypothesis-generating and typically performed to collect and analyze\u00a0<em>all<\/em>\u00a0detectable metabolites in a sample. As a discovery approach, it can reveal novel compounds and chemical unknowns potentially leading to new insights in disease mechanism or treatment.<\/p>\n<p>In an effort to communicate the confidence of annotation and identification results for known features in global, untargeted metabolomics studies, we use an <a href=\"https:\/\/www.vanderbilt.edu\/cit\/metabolite-identification-confidence-levels\/\" target=\"_blank\" rel=\"noopener\">evidence-based classification system<\/a> based on metabolite identification discussions in the metabolomics community.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue8a3' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Targeted Metabolomics<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p><strong>Targeted metabolomics<\/strong>\u00a0enables validation and absolute quantification of a subset of metabolites (e.g., metabolites within a specific pathway). We have several established targeted assay panels available. Please <a href=\"mailto:stacy.d.sherrod@vanderbilt.edu\" target=\"_blank\" rel=\"noopener\">contact us<\/a> for details.<\/p>\n<p>This method can also be used to complement a global, untargeted approach.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue8cd' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Lipidomics<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p>The CIT has developed a conformational lipid atlas containing highly-accurate structural measurements in support of high-confidence lipidomic workflows. This lipid atlas is available in the CIT to help collaborators better understand the role of lipids in cancer biology or other diseases.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue8cc' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Amino Acid Panels<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p>The CIT has developed protocols to profile all 22 proteinogenic amino acids in plasma, serum, urine, cells, and tissue after solvent extraction.\u00a0All analytes and Internal Standards are measured on a high-resolution mass spectrometer using a targeted method and reported as normalized to heavy labeled internal standards.\u00a0Coefficients of variation are generally 10%.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue822' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Data Processing, Analysis, and Interpretation<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p>Our full-service facility provides data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA\/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and metabolite identification services (see explanation of <a href=\"https:\/\/www.vanderbilt.edu\/cit\/metabolite-identification-confidence-levels\/\">metabolite identification confidence levels<\/a>).<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue84d' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Grant Application and Manuscript Support<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p>We are available to provide you with experimental descriptions, high-quality figures, and responses to reviewers.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<li><div  style='background-color:#0472e8; ' class='iconlist_icon  avia-font-entypo-fontello'><span class='iconlist-char ' aria-hidden='true' data-av_icon='\ue856' data-av_iconfont='entypo-fontello'><\/span><\/div><article class=\"article-icon-entry \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='iconlist_content_wrap'><header class=\"entry-content-header\"><h4 class='av_iconlist_title iconlist_title   '  itemprop=\"headline\"  >Custom Method Development<\/h4><\/header><div class='iconlist_content  '  itemprop=\"text\"  ><p>We are flexible and experienced in working with researchers from many different fields for different applications. As a result custom method development is available as needed. Please <a href=\"https:\/\/www.vanderbilt.edu\/cit\/contact-us\/\">contact us<\/a> for more information.<\/p>\n<\/div><\/div><footer class=\"entry-footer\"><\/footer><\/article><div class='iconlist-timeline'><\/div><\/li>\n<\/ul><\/div><\/p><\/div><div class=\"flex_column av_one_fourth  flex_column_div av-zero-column-padding   avia-builder-el-13  el_after_av_three_fourth  el_before_av_button  \" style='border-radius:0px; '><p><div  class='avia-image-container avia_animated_image avia_animate_when_almost_visible pop-up av-styling-    avia-builder-el-14  el_before_av_image  avia-builder-el-first  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='wp-image-116 avia-img-lazy-loading-not-116 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223105\/vials.jpg\" alt='Mass Spectrometry Sample Vials' title='Mass Spectrometry Sample Vials' height=\"570\" width=\"300\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223105\/vials.jpg 300w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223105\/vials-158x300.jpg 158w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/div><\/div><br \/>\n<div  class='avia-image-container avia_animated_image avia_animate_when_almost_visible pop-up av-styling-    avia-builder-el-15  el_after_av_image  el_before_av_image  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='wp-image-117 avia-img-lazy-loading-not-117 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223052\/workstation-300x300.jpg\" alt='Shared Resource Core Workstation' title='Shared Resource Core Workstation' height=\"300\" width=\"300\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223052\/workstation-300x300.jpg 300w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223052\/workstation-80x80.jpg 80w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223052\/workstation-36x36.jpg 36w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223052\/workstation-180x180.jpg 180w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223052\/workstation-120x120.jpg 120w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223052\/workstation-450x452.jpg 450w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223052\/workstation.jpg 550w\" sizes=\"(max-width: 300px) 100vw, 300px\" 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